ELISA/assay

Human bone morphogenetic protein 4, BMP4 ELISA Kit

MBS702135

96 Strip Wells

760 USD

ELISA Kit

Human bone morphogenetic protein 4, BMP4 ELISA Kit

bone morphogenetic protein 4

Human bone morphogenetic protein 4 (BMP4) ELISA Kit; BMP2B; BMP2B1; MCOPS6; OFC11; ZYME; bone morphogenetic protein 2B; bone morphogenetic protein 4

bone morphogenetic protein 4 preproprotein; Bone morphogenetic protein 4; bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B; bone morphogenetic protein 4; Bone morphogenetic protein 2B; BMP-2B

BMP4

BMP4; BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; BMP2B; DVR4; BMP-4; BMP-2B

BMP4_HUMAN

Human

408

This assay has high sensitivity and excellent specificity for detection of Human BMP4. No significant cross-reactivity or interference between Human BMP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Chondrogenesis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

157276593

NP_001193.2

NM_001202.3

P12644

46,555 Da

Adipogenesis Pathway 198832!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Cardiac Progenitor Differentiation Pathway 712094!!Elastic Fibre Formation Pathway 730310!!Extracellular Matrix Organization Pathway 576262!!Glypican 3 Network Pathway 138084!!Heart Development Pathway 198802!!Hedgehog Signaling Pathway 83063!!Hedgehog Signaling Pathway 474

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction

96 Strip Wells

760 USD