Human glutamic acid decarboxylase 65, GAD65 ELISA Kit | MyBioSource MBS702132 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human glutamic acid decarboxylase 65, GAD65 ELISA Kit

catalog :

MBS702132

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702132

products type :

ELISA Kit

products full name :

Human glutamic acid decarboxylase 65, GAD65 ELISA Kit

products short name :

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

products name syn :

Human glutamic acid decarboxylase 65; GAD65 ELISA Kit; RP11-420F12.2; GAD65; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas) ; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain; 65kDa)

other names :

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

products gene name :

GAD2

other gene names :

GAD2; GAD2; GAD65; GAD65; GAD-65

uniprot entry name :

DCE2_HUMAN

reactivity :

Human

sequence length :

585

specificity :

This assay has high sensitivity and excellent specificity for detection of human GAD65 . No significant cross-reactivity or interference between human GAD65 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human GAD65 and is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503875

ncbi acc num :

NP_000809.1

ncbi gb acc num :

NM_000818.2

uniprot acc num :

Q05329

ncbi mol weight :

65,411 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

uniprot summary :

Function: Catalyzes the production of GABA. Catalytic activity: L-glutamate = 4-aminobutanoate + CO2. Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.10

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage