catalog number :
MBS702121
products type :
ELISA Kit
products full name :
Rat Parathyroid hormone, PTH ELISA Kit
products short name :
parathyroid hormone
products name syn :
Rat Parathyroid hormone; PTH ELISA Kit; PTH1; parathormone; parathyrin; parathyroid hormone 1; parathyroid hormone
other names :
parathyroid hormone; Parathyroid hormone; parathyroid hormone; parathyrin; hypothalamic parathyroid hormone; parathyroid hormone; Parathyrin
other gene names :
Pth; Pth; Pth1; Pthr1; PTH-(1-84); PTH
uniprot entry name :
PTHY_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat PTH. No significant cross-reactivity or interference between Rat PTH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: 1.56 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_058740.1
ncbi gb acc num :
NM_017044.1
ncbi mol weight :
12,722 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway (1010189); Endochondral Ossification Pathway (219771); G Alpha (s) Signalling Events Pathway (1010194); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Osteoblast Pathway (198455); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)
ncbi summary :
regulates osteoblast proliferation and differentiation; involved in G-protein coupled receptor mediated adenylate cyclase activation [RGD, Feb 2006]
uniprot summary :
Function: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells
size4 :
10x96-Strip-Wells