ELISA/assay

Rat matrix metalloproteinase 13, MMP-13 ELISA Kit

MBS702112

48-Strip-Wells

465 USD

ELISA Kit

Rat matrix metalloproteinase 13, MMP-13 ELISA Kit

matrix metallopeptidase 13 (collagenase 3)

Rat matrix metalloproteinase 13; MMP-13 ELISA kit; CLG3; MANDP1; collagenase 3; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3) ; matrix metallopeptidase 13 (collagenase 3)

collagenase 3; Collagenase 3; collagenase 3; MMP-13; collagenase-3; matrix metalloproteinase-13; Matrix metalloproteinase-13; MMP-13

MMP13

MMP13; MMP13; MMP-13

MMP13_RABIT

Rat

471

This assay has high sensitivity and excellent specificity for detection of rat MMP-13. No significant cross-reactivity or interference between rat MMP-13 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 93.75 pg/ml -6000 pg/ml. Sensitivity: 23.44 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

126723537

NP_001075506.1

NM_001082037.1

O62806

53,693 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion . Cofactor: Calcium. Can bind about 5 calcium ions per subunit

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595