Mouse apolipoprotein A1, Apo-A1 ELISA Kit | MyBioSource MBS702111 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse apolipoprotein A1, Apo-A1 ELISA Kit

catalog :

MBS702111

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702111

products type :

ELISA Kit

products full name :

Mouse apolipoprotein A1, Apo-A1 ELISA Kit

products short name :

apolipoprotein A-I

products name syn :

Mouse apolipoprotein A1 (Apo-A1) ELISA Kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; apolipoprotein A-I

other names :

apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apolipoprotein A1; apolipoprotein A-I; Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I

products gene name :

APOA1

other gene names :

Apoa1; Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I; Apo-AI; ApoA-I; ProapoA-I

uniprot entry name :

APOA1_MOUSE

reactivity :

Mouse

sequence length :

264

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse Apo-A1. No significant cross-reactivity or interference between mouse Apo-A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: The minimum detectable dose of mouse Apo-A1 is typically less than 7.81 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apo-A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

160333304

ncbi acc num :

NP_033822.2

ncbi gb acc num :

NM_009692.4

uniprot acc num :

Q00623

ncbi mol weight :

30,616 Da

ncbi pathways :

ABC-family Proteins Mediated Transport Pathway (1001328); ABCA Transporters In Lipid Homeostasis Pathway (1001329); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Amyloids Pathway (1000612); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1000591); Chylomicron-mediated Lipid Transport Pathway (1001042); Disease Pathway (970606); Diseases Associated With Visual Transduction Pathway (971454); Fat Digestion And Absorption Pathway (194386)

ncbi summary :

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

uniprot summary :

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage