ELISA/assay

Human deoxypyridinoline, DPD ELISA Kit

MBS702103

48-Strip-Wells

565 USD

ELISA Kit

Human deoxypyridinoline, DPD ELISA Kit

deoxypyridinoline, DPD

Human deoxypyridinoline; DPD ELISA Kit; deoxypyridinoline; DPD

dihydropyrimidine dehydrogenase; Dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; DPD; DHPDHase; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase; dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

DPD

Dpyd; Dpyd; DPD; DHPDHase; DPD

DPYD_RAT

Human

1025

This assay has high sensitivity and excellent specificity for detection of human DPD. No significant cross-reactivity or interference between human DPD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, urine, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPD bound in the initial step. The color development is stopped and the intensity of the color is measured.

13591940

NP_112289.1

NM_031027.1

O89000

111,468 Da

Drug Metabolism - Other Enzymes Pathway (83425); Drug Metabolism - Other Enzymes Pathway (428); Pantothenate And CoA Biosynthesis Pathway (83407); Pantothenate And CoA Biosynthesis Pathway (401); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (606001); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (468239); Pyrimidine Metabolism Pathway (83339); Pyrimidine Metabolism Pathway (309); Beta-Alanine Metabolism Pathway (83358); Beta-Alanine Metabolism Pathway (335)

catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]

Function: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine . Catalytic activity: 5,6-dihydrouracil + NADP+ = uracil + NADPH. Cofactor: Binds 4 4Fe-4S clusters. Contains approximately 16 iron atoms per subunit . Enzyme regulation: Inactivated by 5-iodouracil

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150