Human Glypican-3, GPC-3 ELISA Kit | MyBioSource MBS702092 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glypican-3, GPC-3 ELISA Kit

catalog :

MBS702092

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702092

products type :

ELISA Kit

products full name :

Human Glypican-3, GPC-3 ELISA Kit

products short name :

glypican 3

products name syn :

Human Glypican-3; GPC-3 ELISA Kit; AC002420.1; DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; glypican 3

other names :

glypican-3 isoform 1; Glypican-3; glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7Cleaved into the following chain:Secreted glypican-3

products gene name :

GPC3

other gene names :

GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5

uniprot entry name :

GPC3_HUMAN

reactivity :

Human

sequence length :

603

specificity :

This assay has high sensitivity and excellent specificity for detection of Human GPC3. No significant cross-reactivity or interference between Human GPC3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, urine, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique.Antibody specific for GPC-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPC-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPC-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPC-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

257471006

ncbi acc num :

NP_001158089.1

ncbi gb acc num :

NM_001164617.1

uniprot acc num :

P51654

ncbi mol weight :

65,563 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Glypican 3 Network Pathway (138084); Glypican Pathway (138083); HS-GAG Biosynthesis Pathway (645306); HS-GAG Degradation Pathway (645307)

ncbi summary :

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

uniprot summary :

Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.1 PublicationManual assertion based on experiment in:Ref.8

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage