ELISA/assay

Rat Adipose Triglyceride Lipase, ATGL ELISA Kit

MBS702087

96 Strip Wells

760 USD

ELISA Kit

Rat Adipose Triglyceride Lipase, ATGL ELISA Kit

patatin-like phospholipase domain containing 2

Rat Adipose Triglyceride Lipase; ATGL ELISA Kit; 1110001C14Rik; ATGL; DESNUTRIN; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2; adipose triglyceride lipase; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; patatin-like phospholipase domain containing 2

patatin-like phospholipase domain-containing protein 2; Patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2; adipose triglyceride lipase; patatin-like phospholipase domain containing 2; Adipose triglyceride lipase

PNPLA2

Pnpla2; Pnpla2; RGD1309044; Atgl

PLPL2_RAT

Rat

478

This assay has high sensitivity and excellent specificity for detection of rat ATGL. No significant cross-reactivity or interference between rat ATGL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat adipose triglyceride lipase (ATGL) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml.

Sensitivity: The minimum detectable dose of rat ATGL is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATGL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATGL bound in the initial step. The color development is stopped and the intensity of the color is measured.

189095277

NP_001101979.2

NM_001108509.2

P0C548

52,567 Da

Acyl Chain Remodeling Of DAG And TAG Pathway 1010737!!Acylglycerol Degradation Pathway 434704!!Acylglycerol Degradation Pathway 468272!!Beta Oxidation Meta Pathway 198526!!Fatty Acid Beta Oxidation Pathway 219782!!Fatty Acid Beta Oxidation 1 Pathway 198531!!Glycerolipid Metabolism Pathway 83378!!Glycerolipid Metabolism Pathway 361!!Glycerophospholipid Biosynthesis Pathway 1010735!!Metabolism Pathway 1010675

Function: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion . Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.

96 Strip Wells

760 USD