product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Macrophage Migration Inhibitory Factor, MIF ELISA Kit
catalog :
MBS702084
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS702084
products type :
ELISA Kit
products full name :
Human Macrophage Migration Inhibitory Factor, MIF ELISA Kit
products short name :
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
products name syn :
Human Macrophage Migration Inhibitory Factor; MIF ELISA Kit; GIF; GLIF; MMIF; macrophage migration inhibitory factor; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor)
other names :
macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
products gene name :
MIF
other gene names :
MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF
uniprot entry name :
MIF_HUMAN
reactivity :
Human
sequence length :
115
specificity :
This assay has high sensitivity and excellent specificity for detection of human MIF. No significant cross-reactivity or interference between human MIF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: 31.25 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
4505185
ncbi acc num :
NP_002406.1
ncbi gb acc num :
NM_002415.1
uniprot acc num :
P14174
ncbi mol weight :
12,476 Da
ncbi pathways :
Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)
ncbi summary :
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.3 PublicationsManual assertion based on experiment in:Ref.23. Catalytic activity: Keto-phenylpyruvate = enol-phenylpyruvate.3 PublicationsManual assertion based on experiment in:Ref.33
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!