catalog number :
MBS702077
products type :
ELISA Kit
products full name :
Human proteinase 3, PR3 ELISA Kit
products short name :
proteinase 3
products name syn :
Human proteinase 3 (PR3) ELISA kit; ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP4; P29; PR-3; PR3; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; serine proteinase; neutrophil; proteinase 3
other names :
myeloblastin; Myeloblastin; myeloblastin; C-ANCA antigen; wegener autoantigen; leukocyte proteinase 3; neutrophil proteinase 4; azurophil granule protein 7; serine proteinase, neutrophil; Wegener granulomatosis autoantigen; proteinase 3; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen
products gene name :
PRTN3
other gene names :
PRTN3; PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA; MBN; PR-3; PR3; NP-4
uniprot entry name :
PRTN3_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human PR3. No significant cross-reactivity or interference between human PR3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 0.78 ng/ml -50 ng/ml. Sensitivity: 0.195 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PR3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002768.3
ncbi gb acc num :
NM_002777.3
ncbi mol weight :
27,807 Da
ncbi pathways :
C-MYB Transcription Factor Network Pathway (138073)
uniprot summary :
Function: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala- -Xaa- > -Val- -Xaa-.1 PublicationManual assertion based on experiment in:Ref.18
size4 :
10x96-Strip-Wells