ELISA/assay

Human ribonuclease pancreatic, RNASE1/RIB1/RNS1 ELISA Kit

MBS702071

96 Strip Wells

850 USD

ELISA Kit

Human ribonuclease pancreatic, RNASE1/RIB1/RNS1 ELISA Kit

ribonuclease, RNase A family, 1 (pancreatic)

Human ribonuclease pancreatic (RNASE1/RIB1/RNS1) ELISA kit; MGC12408; RIB1; RNS1; RNase upI-1; pancreatic ribonuclease; ribonuclease; RNase A family; 1 (pancreatic)

ribonuclease pancreatic; Ribonuclease pancreatic; ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A C1; ribonuclease, RNase A family, 1 (pancreatic); HP-RNase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A; RNase A

RNASE1

RNASE1; RNASE1; RAC1; RIB1; RNS1; RIB1; RNS1; RNase 1; RNase A

RNAS1_HUMAN

Human

156

This assay has high sensitivity and excellent specificity for detection of Human RNASE1. No significant cross-reactivity or interference between Human RNASE1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell lysates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNASE1/RIB1/RNS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE1/RIB1/RNS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE1/RIB1/RNS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE1/RIB1/RNS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506547

NP_002924.1

NM_002933.4

P07998

17,644 Da

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Function: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.1 PublicationManual assertion based on experiment in:Ref.19. Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

96 Strip Wells

850 USD