Human immunoglobulin binding protein, BIP ELISA Kit | MyBioSource MBS702067 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human immunoglobulin binding protein, BIP ELISA Kit

catalog :

MBS702067

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702067

products type :

ELISA Kit

products full name :

Human immunoglobulin binding protein, BIP ELISA Kit

products short name :

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

products name syn :

Human immunoglobulin binding protein; BIP ELISA KIT; BIP; FLJ26106; GRP78; MIF2; Heat-shock 70kD protein-5 (glucose-regulated protein; 78kD) ; heat shock 70kD protein 5 (glucose-regulated protein; 78kD) ; heat shock 70kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa)

other names :

78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

products gene name :

HSPA5

other gene names :

HSPA5; HSPA5; BIP; MIF2; GRP78; HEL-S-89n; GRP78; GRP-78; BiP

uniprot entry name :

GRP78_HUMAN

reactivity :

Human

sequence length :

654

specificity :

This assay has high sensitivity and excellent specificity for detection of Human HSPA5. No significant cross-reactivity or interference between Human HSPA5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

16507237

ncbi acc num :

NP_005338.1

ncbi gb acc num :

NM_005347.4

uniprot acc num :

P11021

ncbi mol weight :

72,333 Da

ncbi pathways :

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Hemostasis Pathway (106028); IRE1alpha Activates Chaperones Pathway (105906); Immune System Pathway (106386)

ncbi summary :

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

uniprot summary :

Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.3 PublicationsManual assertion based on experiment in:Ref.12

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage