ELISA/assay

Dog Transforming growth factor beta1, TGF-beta1 ELISA Kit

MBS702055

96-Strip-Wells

680 USD

ELISA Kit

Dog Transforming growth factor beta1, TGF-beta1 ELISA Kit

transforming growth factor, beta 1

Dog Transforming growth factor beta1; TGF-beta1 ELISA Kit; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; Dog Transforming growth factor beta1; TGF-beta1

transforming growth factor beta-1; Transforming growth factor beta-1; transforming growth factor beta-1; TGF-beta-1; transforming growth factor beta 1; transforming growth factor-beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease)

TGFB1

TGFB1; TGFB1; TGF-beta-1; LAP

TGFB1_CANFA

Dog

390

This assay has high sensitivity and excellent specificity for detection of Dog TGFB1. No significant cross-reactivity or interference between Dog TGFB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Detection Range: 0.781 ng/ml-50 ng/ml. Sensitivity: 0.747 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cytokine

Introduction: Transforming growth factor beta 1 or TGF-beta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. Some T cells (e.g. regulatory T cells) release TGF-beta1 to inhibit the actions of other T cells. Interleukin 1- and interleukin 2-dependent proliferation of activated T cells, and the activation of quiescent helper T cells and cytotoxic T cells is prevented by the activity of TGF-beta1. Similarly, TGF-beta1 can inhibit the secretion and activity of many other cytokines including interferon-gamma, tumor necrosis factor-alpha (TNF-alpha) and various interleukins. It can also decrease the expression levels of cytokine receptors, such as the IL-2 receptor to down-regulate the activity of immune cells. However, TGF-beta1 can also increase the expression of certain cytokines in T cells and promote their proliferation, particularly if the cells are immature. TGF-beta1 has similar effects on B cells that also vary according to the differentiation state of the cell. It inhibits proliferation and apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. The effects of TGF-beta1 on macrophages and monocytes are predominantly suppressive; this cytokine can inhibit the proliferation of these cells and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta1 can also have the opposite effect on cells of myeloid origin. For example, TGF-beta1 acts as a chemoattractant, directing an immune response to some pathogens; macrophages and monocytes respond to low levels of TGF-beta1 in a chemotactic manner. Furthermore, the expression of monocytic cytokines and phagocytic killing by macrophages can be increased by the action of TGF-beta1. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for TGF-beta1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

50979134

NP_001003309.1

NM_001003309.1

P54831

44,185 Da

Amoebiasis Pathway (167342); Amoebiasis Pathway (167191); Cell Cycle Pathway (1016974); Cell Cycle, Mitotic Pathway (1016994); Cell Cycle Pathway (920948); Cell Cycle Pathway (84104); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147812); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (84162)

Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts

96-Strip-Wells

680 USD

5x96-Strip-Wells

2140

10x96-Strip-Wells

3990