Human cluster of differentiation 63, CD63 ELISA Kit | MyBioSource MBS702053 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human cluster of differentiation 63, CD63 ELISA Kit

catalog :

MBS702053

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702053

products type :

ELISA Kit

products full name :

Human cluster of differentiation 63, CD63 ELISA Kit

products short name :

CD63 molecule

products name syn :

Human cluster of differentiation 63; CD63 ELISA Kit; LAMP-3; ME491; MLA1; OMA81H; TSPAN30; CD63 antigen; CD63 antigen (melanoma 1 antigen) ; granulophysin; lysosome-associated membrane glycoprotein 3; melanoma 1 antigen; melanoma-associated antigen ME491; me; CD63 molecule

other names :

CD63 antigen isoform A; CD63 antigen; CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; CD63 molecule; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD_antigen: CD63

products gene name :

CD63

other gene names :

CD63; CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; MLA1; TSPAN30; LAMP-3; Tspan-30

uniprot entry name :

CD63_HUMAN

reactivity :

Human

sequence length :

238

specificity :

This assay has high sensitivity and excellent specificity for detection of human CD63. No significant cross-reactivity or interference between human CD63 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human CD63 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD63 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD63 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

383872447

ncbi acc num :

NP_001244318.1

ncbi gb acc num :

NM_001257389.1

uniprot acc num :

P08962

ncbi mol weight :

25,637 Da

ncbi pathways :

Hemostasis Pathway (106028); Lysosome Pathway (99052); Lysosome Pathway (96865); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

uniprot summary :

Function: Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.5 PublicationsManual assertion based on experiment in:Ref.18

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage