ELISA/assay

Human cyclophilin A, PPIA/CYPA ELISA Kit

MBS702050

96 Strip Wells

760 USD

ELISA Kit

Human cyclophilin A, PPIA/CYPA ELISA Kit

peptidylprolyl isomerase A (cyclophilin A)

Human cyclophilin A (PPIA/CYPA) ELISA Kit; CYPA; CYPH; MGC117158; MGC12404; MGC23397; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase A; peptidylprolyl isomerase A (cyclophilin A)

peptidyl-prolyl cis-trans isomerase A isoform 1; Peptidyl-prolyl cis-trans isomerase A; peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; T cell cyclophilin; cyclosporin A-binding protein; epididymis secretory sperm binding protein Li 69p; peptidylprolyl isomerase A (cyclophilin A); Cyclophilin A; Cyclosporin A-binding protein; Rotamase ACleaved into the following chain:Peptidyl-prolyl cis-trans isomerase A, N-terminally processed

PPIA

PPIA; PPIA; CYPA; CYPH; HEL-S-69p; CYPA; PPIase A

PPIA_HUMAN

Human

165

This assay has high sensitivity and excellent specificity for detection of Human PPIA. No significant cross-reactivity or interference between Human PPIA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 3.12 ng/ml-200 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Host-virus interaction

Introduction: Cyclophilins, abbreviated Cyp, are proteins that bind to cyclosporine (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants. These proteins have peptidyl prolyl isomerase activity, which catalyzes the isomerization of peptide bonds from trans form to cis form at proline residues and facilitates protein folding. Cyclophilin A (CyPA) is an abundant intracellular protein that is considered to be the main target of the immunosuppressive drug cyclosporine A. Human Cyclophilin A exists in cytosol, has a beta barrel structure with two alpha helices and a beta-sheet. Other cyclophilins have similar structures to cyclophilin A. The cyclosporin A-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CyPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for CyPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CyPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CyPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10863927

NP_066953.1

NM_021130.4

P62937

18,012 Da

APOBEC3G Mediated Resistance To HIV-1 Infection Pathway 106016!!Assembly Of The HIV Virion Pathway 106008!!Basigin Interactions Pathway 106065!!Binding And Entry Of HIV Virion Pathway 105986!!Budding And Maturation Of HIV Virion Pathway 106009!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Disease Pathway 530764!!Early Phase Of HIV Life Cycle Pathway 105985!!HIV Infection Pathway 105983!!HIV Life Cycle Pathway 105984

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0). Enzyme regulation: Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.

96 Strip Wells

760 USD