ELISA/assay

Human Coagulation Factor XIII A1 Polypeptide, F13A1 ELISA Kit

MBS702048

96 Strip Wells

850 USD

ELISA Kit

Human Coagulation Factor XIII A1 Polypeptide, F13A1 ELISA Kit

coagulation factor XIII, A1 polypeptide

Human Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA kit; RP11-232H4.1; F13A; FSF; A subunit; TGase; bA525O21.1 (coagulation factor XIII; A1 polypeptide) ; coagulation factor XIII A1 subunit; coagulation factor XIII; A polypeptide; factor XIIIa; fibrin stabilizin; coagulation factor XIII; A1 polypeptide

coagulation factor XIII A chain; Coagulation factor XIII A chain; coagulation factor XIII A chain; TGase; factor XIIIa; fibrinoligase; FSF, A subunit; coagulation factor XIIIa; transglutaminase A chain; transglutaminase. plasma; fibrin stabilizing factor, A subunit; coagulation factor XIII, A polypeptide; protein-glutamine gamma-glutamyltransferase A chain; bA525O21.1 (coagulation factor XIII, A1 polypeptide); coagulation factor XIII, A1 polypeptide; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain

F13A1

F13A1; F13A1; F13A; F13A; Coagulation factor XIIIa

F13A_HUMAN

Human

732

This assay has high sensitivity and excellent specificity for detection of Human F13A1. No significant cross-reactivity or interference between Human F13A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 62.5 pg/ml-4000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F13A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F13A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F13A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F13A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

119395709

NP_000120.2

NM_000129.3

P00488

83,267 Da

Common Pathway 106060!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!Hemostasis Pathway 106028!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Function: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Catalytic activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3. Cofactor: Binds 1 calcium ion per subunit.

96 Strip Wells

850 USD