ELISA/assay

Mouse Macrophage-Derived Chemokine, MDC ELISA Kit

MBS702047

96 Strip Wells

655 USD

ELISA Kit

Mouse Macrophage-Derived Chemokine, MDC ELISA Kit

chemokine (C-C motif) ligand 22

Mouse Macrophage-Derived Chemokine (MDC/CCL22) ELISA kit; A-152E5.1; ABCD-1; DC/B-CK; MDC; MGC34554; SCYA22; STCP-1; CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys); member 22; sti; Mouse Macrophage-Derived Chemokine (MDC/CCL22)

C-C motif chemokine 22; C-C motif chemokine 22; C-C motif chemokine 22; CC chemokine ABCD-1; small-inducible cytokine A22; activated B and dendritic cell-derived; small inducible cytokine subfamily A22; dendritic cell and B cell derived chemokine; small inducible cytokine subfamily A, member 22; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED); chemokine (C-C motif) ligand 22; Activated B and dendritic cell-derived; CC chemokine ABCD-1; Small-inducible cytokine A22

CCL22

Ccl22; Ccl22; MDC; DCBCK; ABCD-1; Scya22; Abcd1; Scya22

CCL22_MOUSE

Mouse

92

This assay has high sensitivity and excellent specificity for detection of mouse MDC. No significant cross-reactivity or interference between mouse MDC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of mouse macrophage-derived chemokine (MDC) concentrations in serum, plasma, tissue homogenates, cell culture supernates, cell lysates. Detection Range: 31.25 pg/ml -2000 pg/ml.

Sensitivity: The minimum detectable dose of mouse MDC is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MDC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDC bound in the initial step. The color development is stopped and the intensity of the color is measured.

6677879

NP_033163.1

NM_009137.2

O88430

10,302 Da

Chemokine Receptors Bind Chemokines Pathway 1000944!!Chemokine Signaling Pathway 99271!!Chemokine Signaling Pathway 672435!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!GPCR Ligand Binding Pathway 1000941!!Peptide Ligand-binding Receptors Pathway 1000943!!Signal Transduction Pathway 970540

Function: Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.

96 Strip Wells

655 USD