Rat Insulin-like growth factor 2, IGF-2 ELISA Kit | MyBioSource MBS702040 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Insulin-like growth factor 2, IGF-2 ELISA Kit

catalog :

MBS702040

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702040

products type :

ELISA Kit

products full name :

Rat Insulin-like growth factor 2, IGF-2 ELISA Kit

products short name :

insulin-like growth factor 2 (somatomedin A)

products name syn :

Rat Insulin-like growth factor 2; IGF-2 ELISA Kit; C11orf43; FLJ22066; FLJ44734; INSIGF; pp9974; OTTHUMP00000011018; OTTHUMP00000011157; insulin-like growth factor 2; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like; insulin-like growth factor 2 (somatomedin A)

other names :

insulin-like growth factor II isoform 2 preproprotein; Insulin-like growth factor II; insulin-like growth factor II; MSA; IGF-II; multiplication-stimulating activity; insulin-like growth factor II (IGF-II); multiplication-stimulating polypeptide; Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein; insulin-like growth factor 2; Multiplication-stimulating activity; MSA; Multiplication-stimulating polypeptideCleaved into the following 2 chains:Insulin-like growth factor II; Preptin

products gene name :

IGF2

other gene names :

Igf2; Igf2; IGFII; RNIGF2; Igf-2; IGF-II; MSA

uniprot entry name :

IGF2_RAT

reactivity :

Rat

sequence length :

180

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat IGF2. No significant cross-reactivity or interference between Rat IGF2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Carbohydrate metabolism

products description :

Introduction: Insulin-like growth factor 2 (IGF-2) is one of three protein hormones that share structural similarity to insulin. In humans, the IGF2 gene is located on chromosome 11p15.5, a region which contains numerous imprinted genes. In mice this homologous region is found at distal chromosome 7. In both organisms, Igf2 is imprinted, with expression resulting favourably from the paternally inherited allele. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for IGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

297747332

ncbi acc num :

NP_001177091.1

ncbi gb acc num :

NM_001190162.1

uniprot acc num :

P01346

ncbi mol weight :

20,086 Da

ncbi pathways :

Apoptosis Pathway (219785); Endochondral Ossification Pathway (219771); Hedgehog Signaling Pathway (198499); Hemostasis Pathway (1010891); IGF1R Signaling Cascade Pathway (1010238); IRS-related Events Triggered By IGF1R Pathway (1010240); Metabolism Of Proteins Pathway (1009706); Platelet Activation, Signaling And Aggregation Pathway (1010901); Platelet Degranulation Pathway (1010913); Proteoglycans In Cancer Pathway (782034)

ncbi summary :

a mitogenic growth factor; may have a role in fetal development [RGD, Feb 2006]

uniprot summary :

Function: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.1 PublicationManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

615

size3 :

5x96-Strip-Wells

price3 :

2340

size4 :

10x96-Strip-Wells

price4 :

4370

more info or order :

MyBioSource product webpage