ELISA/assay

Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

MBS702037

96 Strip Wells

850 USD

ELISA Kit

Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

Human Angiotensin converting enzyme 2; ACE2 ELISA Kit; ACEH; DKFZp434A014; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin converting enzyme-like protein; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

angiotensin-converting enzyme 2; Angiotensin-converting enzyme 2; angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15Cleaved into the following chain:Processed angiotensin-converting enzyme 2

ACE2

ACE2; ACE2; ACEH; UNQ868/PRO1885; ACEH

ACE2_HUMAN

Human

805

This assay has high sensitivity and excellent specificity for detection of Human ACE2. No significant cross-reactivity or interference between Human ACE2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Host-virus interaction

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

11225609

NP_068576.1

NM_021804.2

Q9BYF1

92,463 Da

ACE Inhibitor Pathway 198763!!Metabolism Of Angiotensinogen To Angiotensins Pathway 685555!!Metabolism Of Proteins Pathway 106230!!Peptide Hormone Metabolism Pathway 771603!!Protein Digestion And Absorption Pathway 172847!!Protein Digestion And Absorption Pathway 171868!!Renin-angiotensin System Pathway 83075!!Renin-angiotensin System Pathway 486

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.4 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine. Cofactor: Binds 1 zinc ion per subunit.1 PublicationManual assertion based on experiment in:Ref.15. Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat.4 PublicationsManual assertion based on experiment in:Ref.1

96 Strip Wells

850 USD