ELISA/assay

Human sonic hedgehog ELISA Kit

MBS702035

48-Strip-Wells

565 USD

ELISA Kit

Human sonic hedgehog ELISA Kit

sonic hedgehog homolog (Drosophila)

Human sonic hedgehog ELISA kit; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog; sonic hedgehog homolog (Drosophila)

sonic hedgehog protein preproprotein; Sonic hedgehog protein; sonic hedgehog protein; sonic hedgehog homolog; sonic hedgehog; HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product; Sonic hedgehog protein C-product

SHH

SHH; SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5; SHH

SHH_HUMAN

Human

462

This assay has high sensitivity and excellent specificity for detection of human sonic hedgehog. No significant cross-reactivity or interference between human sonic hedgehog and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human sonic hedgehog is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sonic hedgehog has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sonic hedgehog present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sonic hedgehog is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sonic hedgehog bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506939

NP_000184.1

NM_000193.2

Q15465

49,607 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); Disease Pathway (530764); FOXA1 Transcription Factor Network Pathway (137979); GPCR Ligand Binding Pathway (161020); Glypican 3 Network Pathway (138084); Heart Development Pathway (198802); Hedgehog Signaling Pathway (198835); Hedgehog Ligand Biogenesis Pathway (1016168)

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

Function: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO 1 PublicationManual assertion based on experiment in:Ref.11

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150