catalog number :
MBS702033
products type :
ELISA Kit
products full name :
Chicken Parathyroid hormone, PTH ELISA Kit
products short name :
parathyroid hormone
products name syn :
Chicken Parathyroid hormone; PTH ELISA Kit; PTH1; parathormone; parathyrin; parathyroid hormone 1; parathyroid hormone
other names :
parathyroid hormone preproprotein; Parathyroid hormone; parathyroid hormone; parathyroid hormone
other gene names :
PTH; PTH; PTH
uniprot entry name :
PTHY_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Chicken PTH. No significant cross-reactivity or interference between Chicken PTH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 6.25pg/ml-100pg/ml. Sensitivity: 3.125pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to PTH has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated PTH and incubated. A competitive inhibition reaction is launched between PTH (Standards or samples) and Biotin-conjugated PTH with the pre-coated antibody specific for PTH. The more amount of PTH in samples, the less antibody bound by Biotin-conjugated PTH. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of PTH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_990783.1
ncbi gb acc num :
NM_205452.2
ncbi mol weight :
13,943 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
size4 :
10x96-Strip-Wells