catalog number :
MBS702014
products type :
ELISA Kit
products full name :
Human desmogleins 1, Dsg-1 ELISA Kit
products short name :
[desmoglein 1]
products name syn :
[Human desmogleins 1; Dsg-1 ELISA Kit; CDHF4; DG1; DSG; PPKS1; SPPK1; desmosomal glycoprotein 1; pemphigus foliaceus antigen; desmoglein 1]
other names :
[desmoglein-1 preproprotein; Desmoglein-1; desmoglein-1; DGI; cadherin family member 4; desmosomal glycoprotein 1; pemphigus foliaceus antigen; desmoglein 1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen]
products gene name :
[DSG1]
other gene names :
[DSG1; DSG1; DG1; DSG; CDHF4; EPKHE; PPKS1; SPPK1; EPKHIA; CDHF4; DG1; DGI]
uniprot entry name :
DSG1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human DNASE1L3. No significant cross-reactivity or interference between human DNASE1L3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001933.2
ncbi gb acc num :
NM_001942.2
ncbi mol weight :
113,748 Da
ncbi pathways :
Apoptosis Pathway (105648); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (105680); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867)
ncbi summary :
Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. The protein encoded by this gene has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells