ELISA/assay

Human Cholesteryl ester transfer protein, CETP ELISA Kit

MBS702011

48-Strip-Wells

565 USD

ELISA Kit

Human Cholesteryl ester transfer protein, CETP ELISA Kit

cholesteryl ester transfer protein, plasma

Human Cholesteryl ester transfer protein; CETP ELISA Kit; HDLCQ10; lipid transfer protein; cholesteryl ester transfer protein; plasma

cholesteryl ester transfer protein isoform 1; Cholesteryl ester transfer protein; cholesteryl ester transfer protein; lipid transfer protein I; BPI fold containing family F; cholesteryl ester transfer protein, plasma; Lipid transfer protein I

CETP

CETP; CETP; BPIFF; HDLCQ10

CETP_HUMAN

Human

493

This assay has high sensitivity and excellent specificity for detection of human CETP. No significant cross-reactivity or interference between human CETP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.7 8 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of human CETP is typically less than 0.195 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CETP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CETP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CETP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CETP bound in the initial step. The color development is stopped and the intensity of the color is measured.

169636439

NP_000069.2

NM_000078.2

P11597

54,756 Da

HDL-mediated Lipid Transport Pathway (106158); LDL-mediated Lipid Transport Pathway (106159); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Statin Pathway (198852); Statin Pathway (198363)

The protein encoded by this gene is found in plasma, where it is involved in the transfer of cholesteryl ester from high density lipoprotein (HDL) to other lipoproteins. Defects in this gene are a cause of hyperalphalipoproteinemia 1 (HALP1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Function: Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150