Human Placental alkaline pkosphatase, PLAP ELISA Kit | MyBioSource MBS701995 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Placental alkaline pkosphatase, PLAP ELISA Kit

catalog :

MBS701995

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Sarker S, Scholz Romero K, Perez A, Illanes S, Mitchell M, Rice G, et al. Placenta-derived exosomes continuously increase in maternal circulation over the first trimester of pregnancy. J Transl Med. 2014;12:204 pubmed publisher

product information

catalog number :

MBS701995

products type :

ELISA Kit

products full name :

Human Placental alkaline pkosphatase, PLAP ELISA Kit

products short name :

alkaline phosphatase, placental (Regan isozyme)

products name syn :

Human Placental alkaline pkosphatase; PLAP ELISA Kit; ALP; FLJ61142; PALP; PLAP; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase; alkaline phosphatase; placental (Regan isozyme)

other names :

alkaline phosphatase, placental type preproprotein; Alkaline phosphatase, placental type; alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

products gene name :

ALPP

other gene names :

ALPP; ALPP; ALP; PALP; PLAP; PLAP-1; PLAP; PLAP-1

uniprot entry name :

PPB1_HUMAN

reactivity :

Human

sequence length :

535

specificity :

This assay has high sensitivity and excellent specificity for detection of human PLAP. No significant cross-reactivity or interference between human PLAP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 84 pg/ml -2000 pg/ml. Sensitivity: 34 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PLAP. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

94721246

ncbi acc num :

NP_001623.3

ncbi gb acc num :

NM_001632.3

uniprot acc num :

P05187

ncbi mol weight :

57,954 Da

ncbi pathways :

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956)

ncbi summary :

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]. Protein type: Membrane protein, integral; Cell surface; Motility/polarity/chemotaxis; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; EC 3.1.3.1. Chromosomal Location of Human Ortholog: 2q37. Cellular Component: cell surface; plasma membrane; integral to membrane. Molecular Function: alkaline phosphatase activity; zinc ion binding; magnesium ion binding. Biological Process: dephosphorylation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage