ELISA/assay

Human apolipoprotein A1, Apo-A1 ELISA Kit

MBS701983

96-Strip-Wells

745 USD

ELISA Kit

Human apolipoprotein A1, Apo-A1 ELISA Kit

apolipoprotein A-I

Human apolipoprotein A1 (Apo-A1) ELISA Kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; Human apolipoprotein A1 (Apo-A1)

apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apo-AI; apolipoprotein A-I; Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-IAlternative name(s):Apolipoprotein A-I(1-242)

APOA1

APOA1; APOA1; Apo-AI; ApoA-I; ProapoA-I

APOA1_HUMAN

Human

267

This assay has high sensitivity and excellent specificity for detection of human Apo-A1. No significant cross-reactivity or interference between human Apo-A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, saliva, urine. Assay Type: Sandwich. Detection Range: 1. 4 ng/ml -1000 ng/ml. Sensitivity: The minimum detectable dose of human Apo-A1 is typically less than 0.7 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for Apo-A1 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557321

NP_000030.1

NM_000039.1

P02647

30,778 Da

ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.1 PublicationManual assertion based on experiment in:Ref.20

96-Strip-Wells

745 USD

5x96-Strip-Wells

2260

10x96-Strip-Wells

4220