ELISA/assay

Human Retinol binding protein 4, RBP-4 ELISA Kit

MBS701969

96 Strip Wells

895 USD

ELISA Kit

Human Retinol binding protein 4, RBP-4 ELISA Kit

retinol binding protein 4, plasma

Human Retinol binding protein 4; RBP-4 ELISA Kit; OTTHUMP00000020116; retinol-binding protein 4; interstitial; retinol-binding protein 4; plasma; retinol binding protein 4; plasma

retinol-binding protein 4; Retinol-binding protein 4; retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial; retinol binding protein 4, plasma; Plasma retinol-binding protein; PRBP; RBP

RBP4

RBP4; RBP4; RDCCAS; PRO2222; PRBP; RBP

RET4_HUMAN

Human

201

This assay has high sensitivity and excellent specificity for detection of Human RBP4. No significant cross-reactivity or interference between Human RBP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, urine, cell culture supernates, tissue homogenates. Detection Range: 0.25 ug/ml-100 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 0.1 ug/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Sensory transduction

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to RBP-4 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated RBP-4 and incubated. A competitive inhibition reaction is launched between RBP-4 (Standards or samples) and HRP-conjugated RBP-4 with the pre-coated antibody specific for RBP-4. The more amount of RBP-4 in samples, the less antibody bound by HRP-conjugated RBP-4. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of RBP-4 in the sample. The color development is stopped and the intensity of the color is measured.

55743122

NP_006735.2

NM_006744.3

P02753

23,010 Da

Disease Pathway 530764!!Diseases Associated With Visual Transduction Pathway 771581!!Retinoid Cycle Disease Events Pathway 771582!!Retinoid Metabolism And Transport Pathway 187208!!SREBP Signalling Pathway 219802!!Signal Transduction Pathway 477114!!The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway 771585!!Visual Phototransduction Pathway 771584!!Vitamin A And Carotenoid Metabolism Pathway 198785!!Retinoate Biosynthesis I Pathway 545452

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

96 Strip Wells

895 USD