ELISA/assay

Mouse Soluble Toll-like Receptor 2, sTLR2 ELISA Kit

MBS701961

96 Strip Wells

850 USD

ELISA Kit

Mouse Soluble Toll-like Receptor 2, sTLR2 ELISA Kit

toll-like receptor 2

Mouse Soluble Toll-like Receptor 2; sTLR2 ELISA Kit; CD282; TIL4; toll/interleukin 1 receptor-like 4; toll-like receptor 2

toll-like receptor 2; Toll-like receptor 2; toll-like receptor 2; toll-like receptor 2; CD_antigen: CD282

TLR2

Tlr2; Tlr2; Ly105

TLR2_MOUSE

Mouse

784

This assay has high sensitivity and excellent specificity for detection of mouse sTLR2. No significant cross-reactivity or interference between mouse sTLR2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of mouse soluble toll-like receptor 2 (sTLR2) concentrations in serum, plasma, tissue homogenates. Detection Range: `0.625 ng/ml -40 ng/ml.

Sensitivity: The minimum detectable dose of mouse sTLR2 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTLR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTLR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTLR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTLR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

158749638

NP_036035.3

NP_036035.3

Q9QUN7

89,449 Da

Amoebiasis Pathway 167330!!Amoebiasis Pathway 167191!!Chagas Disease (American Trypanosomiasis) Pathway 147810!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Hepatitis B Pathway 694729!!Herpes Simplex Infection Pathway 377874!!Herpes Simplex Infection Pathway 377865!!Immune System Pathway 971216!!Inflammatory Bowel Disease (IBD) Pathway 842778!!Inflammatory Bowel Disease (IBD) Pathway 842797

Function: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins 1 PublicationManual assertion based on experiment in:Ref.9

96 Strip Wells

850 USD