ELISA/assay

Human neutrophil activating protein-2, NAP-2 ELISA Kit

MBS701954

96-Strip-Wells

565 USD

ELISA Kit

Human neutrophil activating protein-2, NAP-2 ELISA Kit

pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)

Human neutrophil activating protein-2; NAP-2 ELISA Kit; B-TG1; Beta-TG; CTAP-III; CTAP3; CTAPIII; CXCL7; LA-PF4; LDGF; MDGF; NAP-2; PBP; SCYB7; TC1; TC2; TGB; TGB1; THBGB; THBGB1; CXC chemokine ligand 7; beta-thromboglobulin; connective tissue-activating p; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)

platelet basic protein preproprotein; Platelet basic protein; platelet basic protein; thrombocidin 1; thrombocidin 2; beta-thromboglobulin; CXC chemokine ligand 7; C-X-C motif chemokine 7; thromboglobulin, beta-1; small inducible cytokine B7; small-inducible cytokine B7; leukocyte-derived growth factor; low-affinity platelet factor IV; neutrophil-activating peptide 2; neutrophil-activating peptide-2; macrophage-derived growth factor; connective tissue-activating peptide III; small inducible cytokine subfamily B, member 7; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7Cleaved into the following 10 chains:Connective tissue-activating peptide III; CTAP-IIIAlternative name(s):LA-PF4; Low-affinity platelet factor IV

PPBP

PPBP; PPBP; PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; CTAP-III; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; PBP; LDGF; MDGF; CTAP-III; CTAP-III(1-81); Beta-TG; NAP-2(74); NAP-2(73); NAP-2; NAP-2(1-66); NAP-2(1-63)

CXCL7_HUMAN

Human

128

This assay has high sensitivity and excellent specificity for detection of human NAP-2. No significant cross-reactivity or interference between human NAP-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.45 ng/ml -30 ng/ml. Sensitivity: The minimum detectable dose of human NAP-2 is typically less than 0.11 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4505981

NP_002695.1

NM_002704.3

P02775

13,894 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Hemostasis Pathway (106028)

The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. [provided by RefSeq, May 2010]

Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.4 PublicationsManual assertion based on experiment in:Ref.11

96-Strip-Wells

565 USD

5x96-Strip-Wells

1725

10x96-Strip-Wells

3200