catalog number :
MBS701942
products type :
ELISA Kit
products full name :
Rat Interleukin 15, IL-15 ELISA Kit
products short name :
[interleukin 15]
products name syn :
[Rat Interleukin 15; IL-15 ELISA KIT; IL-15; MGC9721; ; interleukin 15]
other names :
[interleukin-15; Interleukin-15; interleukin-15; IL-15]
products gene name :
[IL15]
other gene names :
[IL15; IL15; IL-15]
uniprot entry name :
IL15_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IL-15. No significant cross-reactivity or interference between rat IL-15 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: 0.39 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075685.1
ncbi gb acc num :
NM_001082216.2
ncbi mol weight :
18,330 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198283); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); Intestinal Immune Network For IgA Production Pathway (128761); Intestinal Immune Network For IgA Production Pathway (128670); Jak-STAT Signaling Pathway (83274)
uniprot summary :
Function: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells