Human Matrix Metalloproteinase 12, MMP-12 ELISA Kit | MyBioSource MBS701937 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Matrix Metalloproteinase 12, MMP-12 ELISA Kit

catalog :

MBS701937

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701937

products type :

ELISA Kit

products full name :

Human Matrix Metalloproteinase 12, MMP-12 ELISA Kit

products short name :

matrix metallopeptidase 12 (macrophage elastase)

products name syn :

Human Matrix Metalloproteinase 12 (MMP-12) ELISA Kit; HME; MGC138506; MME; macrophage elastase; macrophage metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase) ; matrix metallopeptidase 12 (macrophage elastase)

other names :

macrophage metalloelastase preproprotein; Macrophage metalloelastase; macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase); matrix metallopeptidase 12 (macrophage elastase); Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

products gene name :

MMP12

other gene names :

MMP12; MMP12; ME; HME; MME; MMP-12; HME; MME; ME; hME; MMP-12

uniprot entry name :

MMP12_HUMAN

reactivity :

Human

sequence length :

470

specificity :

This assay has high sensitivity and excellent specificity for detection of human MMP-12. No significant cross-reactivity or interference between human MMP-12 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human MMP-12 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

73858572

ncbi acc num :

NP_002417.2

ncbi gb acc num :

NM_002426.4

uniprot acc num :

P39900

ncbi mol weight :

54,002 Da

ncbi pathways :

Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Catalytic activity: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala- -Leu-15 and 16-Tyr- -Leu-17 in the B chain of insulin. Cofactor: Binds 4 calcium ions per subunit.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage