catalog number :
MBS701926
products type :
ELISA Kit
products full name :
Human mucin-5AC, MUC5AC ELISA Kit
products short name :
mucin-5AC
products name syn :
Human mucin-5AC (MUC5AC) ELISA kit; MUC5; gastric mucin; mucin 5; subtypes A and C; tracheobronchial/gastric; mucin 5AC; tracheobronchial mucin; mucin-5AC
other names :
Mucin-5AC; Mucin-5AC; mucin-5AC; mucin 5AC, oligomeric mucus/gel-forming; gastric mucin; tracheobronchial mucin; major airway glycoprotein; lewis B blood group antigen; mucin-5 subtype AC, tracheobronchial; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming; Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; TBM
products gene name :
MUC5AC
other gene names :
MUC5AC; MUC5AC; TBM; leB; MUC5; MUC5; MUC-5AC; LeB; TBM
uniprot entry name :
MUC5A_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MUC5AC. No significant cross-reactivity or interference between human MUC5AC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, urineurineurineurineurine. Assay Type: Sandwich. Detection Range: 15.6 ng/ml-1000 ng/ml. Sensitivity: 3.9 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5AC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5AC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5AC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5AC bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
526,608 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)
uniprot summary :
Function: Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
size4 :
10x96-Strip-Wells