ELISA/assay

Mouse anti-double stranded DNA antibody, IgM ELISA Kit

MBS701925

48-Strip-Wells

510 USD

ELISA Kit

Mouse anti-double stranded DNA antibody, IgM ELISA Kit

anti-double stranded DNA antibody (IgM)

Mouse anti-double stranded DNA antibody (IgM) ELISA Kit; anti-double stranded DNA antibody (IgM)

Mouse

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anti-mouse IgM antibody . Standards or samples are then added to the appropriate microtiter plate wells with biotin-conjugated dsDNA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain anti-mouse IgM antibody, biotin-conjugated dsDNA and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of anti-double stranded DNA antibody(IgM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800