human versican/PG-M/PG-350 ELISA Kit | MyBioSource MBS701920 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

human versican/PG-M/PG-350 ELISA Kit

catalog :

MBS701920

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS701920

products type :

ELISA Kit

products full name :

human versican/PG-M/PG-350 ELISA Kit

products short name :

[versican]

products name syn :

[human versican/PG-M/PG-350 ELISA kit; CSPG2; DKFZp686K06110; ERVR; GHAP; PG-M; WGN; WGN1; chondroitin sulfate proteoglycan 2; glial hyaluronate-binding protein; versican proteoglycan; versican]

other names :

[versican core protein isoform 2; Versican core protein; versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2; versican; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan 2; Glial hyaluronate-binding protein; GHAP; Large fibroblast proteoglycan; PG-M]

products gene name :

[VCAN]

other gene names :

[VCAN; VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2; CSPG2; Chondroitin sulfate proteoglycan 2; GHAP]

uniprot entry name :

CSPG2_HUMAN

reactivity :

Human

sequence length :

655

specificity :

This assay has high sensitivity and excellent specificity for detection of Human VCAN. No significant cross-reactivity or interference between Human VCAN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, cell culture supernates, tissue homogenates, Cell lysates. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Introduction: Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. In 1989, Zimmermann and Ruoslahti cloned and sequenced the core protein of fibroblast chondroitin sulfate proteoglycan. They designated it versican in recognition of its versatile modular structure. Versican belongs to the lectican protein family, with Aggrecan (abundant in Cartilage), Brevican and Neurocan (nervous system proteoglycans) as other members. Versican is also known as Chondroitin sulfate proteoglycan core protein 2 (CSPG-2), PG-M, and Chondroitin sulfate proteoglycan 2. The role of versican in cell adhesion, migration, and proliferation is extensively studied. Versican is often considered an anti-adhesive molecule. Considering the large size (>1000 kDa) and hydration capability of versican, it is possible that the interaction of integrins (large family of cell adhesion molecules) with their cell surface receptors is sterically hindered. Expression of versican is observed in various adult tissues such as blood vessels, skin, and developing heart. Smooth muscle cells of blood vessels, epithelial cells of skin, and the cells of central and peripheral nervous system are a few examples of cell types that express versican physiologically. Versican is involved in development, guiding embryonic cell migration important in the formation of the heart and outlining the path for neural crest cell migration. Versican is a key factor in inflammation through interactions with adhesion molecules on the surfaces of inflammatory leukocytes and interactions with chemokines that are involved in recruiting inflammatory cells. Increased versican expression is often observed in tumor growth in tissues such as breast, brain, ovary, gastrointestinal tract, prostate, and melanoma, Sarcoma, and mesothelioma. In the adult central nervous system, versican is found in perineuronal nets, where it may stabilize synaptic connections. Versican can also inhibit nervous system regeneration and axonal growth following an injury to the central nervous system. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to versican. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for versican and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain versican, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of versican in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187607300

ncbi acc num :

NP_001119808.1

ncbi gb acc num :

NM_001126336.2

uniprot acc num :

P13611

ncbi mol weight :

372,820 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); CS/DS Degradation Pathway (645311); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate Biosynthesis Pathway (645309); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Biosynthesis Pathway (645310); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); ECM Proteoglycans Pathway (833812)

ncbi summary :

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

uniprot summary :

Function: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145