catalog number :
MBS701908
products type :
ELISA Kit
products full name :
Rat Catalase, Cat ELISA Kit
products short name :
[catalase]
products name syn :
[Rat Catalase (CAT) ELISA kit; MGC138422; MGC138424; ; catalase]
other names :
[catalase; Catalase; catalase; catalase]
products gene name :
[CAT]
other gene names :
[Cat; Cat; CS1; Cas1; Catl; Cs-1; Cat01; Cas1]
uniprot entry name :
CATA_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CAT. No significant cross-reactivity or interference between rat CAT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates. Detection Range: 93.75 mIU/ml -6000 mIU/mL. Sensitivity: <23.43 mIU/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036652.1
ncbi gb acc num :
NM_012520.2
ncbi mol weight :
59,757 Da
ncbi pathways :
Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (1010374); Detoxification Of Reactive Oxygen Species Pathway (1010380); Folic Acid Network Pathway (219799); FoxO Signaling Pathway (921494); Glyoxylate And Dicarboxylate Metabolism Pathway (83394); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Metabolism Pathway (1010675); Metabolism Of Nucleotides Pathway (1010808)
ncbi summary :
functions as a hydrogen peroxide:hydrogen peroxide reductase [RGD, Feb 2006]
uniprot summary :
Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells. Catalytic activity: 2 H2O2 = O2 + 2 H2O. Cofactor: Heme group.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells