catalog number :
MBS701901
products type :
ELISA Kit
products full name :
Bovine Fibronectin, FN ELISA Kit
products short name :
fibronectin 1
products name syn :
Bovine Fibronectin; FN ELISA Kit; CIG; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; OTTHUMP00000206762; OTTHUMP00000206767; cold-insoluble globulin; migration-stimulating fa; fibronectin 1
other names :
Fibronectin; Fibronectin; fibronectin; fibronectin ED-A- region; fibronectin variable region; embryo-specific fibronectin 1 transcript; cumulus cell-specific fibronectin 1 transcript; fibronectin 1
other gene names :
FN1; FN1; FN; FN
uniprot entry name :
FINC_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine FN1. No significant cross-reactivity or interference between Bovine FN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates and Cell lysates. Detection Range: 1.56 ug/ml-100 ug/ml. Sensitivity: 0.39 ug/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Acute phase
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for FN to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FN, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi mol weight :
272,154 Da
ncbi pathways :
Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149789); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (1009074); ECM-receptor Interaction Pathway (84307); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1008838); Fibronectin Matrix Formation Pathway (1008844); Focal Adhesion Pathway (84306)
uniprot summary :
Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts
size4 :
10x96-Strip-Wells