ELISA/assay

Mouse 5-Nucleotidase, 5-NT ELISA Kit

MBS701898

48-Strip-Wells

435 USD

ELISA Kit

Mouse 5-Nucleotidase, 5-NT ELISA Kit

5-Nucleotidase, 5-NT

Mouse 5-Nucleotidase; 5-NT ELISA Kit; 5-Nucleotidase; 5-NT

5'-nucleotidase isoform 2 preproprotein; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase; 5'-nucleotidase, ecto (CD73); Ecto-5'-nucleotidase; CD_antigen: CD73

5-NT

NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; NT5; NTE; 5'-NT

5NTD_HUMAN

Mouse

524

This assay has high sensitivity and excellent specificity for detection of mouse 5-NT. No significant cross-reactivity or interference between mouse 5-NT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.125 mU/ml -8 mU/ml. Sensitivity: 0.031 mU/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

325651886

NP_001191742.1

NM_001204813.1

P21589

57,949 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); NAD Salvage Pathway II (545284); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265)

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. Cofactor: Zinc.

48-Strip-Wells

435 USD

96-Strip-Wells

615

5x96-Strip-Wells

2340

10x96-Strip-Wells

4370