Human Triggering Receptor Expresses on Myeloid Cells-1, TREM-1 ELISA Kit | MyBioSource MBS701880 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Triggering Receptor Expresses on Myeloid Cells-1, TREM-1 ELISA Kit

catalog :

MBS701880

quantity :

96 Strip Wells

price :

670 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701880

products type :

ELISA Kit

products full name :

Human Triggering Receptor Expresses on Myeloid Cells-1, TREM-1 ELISA Kit

products short name :

triggering receptor expressed on myeloid cells 1

products name syn :

Human Triggering Receptor Expresses on Myeloid Cells-1; TREM-1 ELISA Kit; TREM-1; triggering-receptor TREM1; triggering receptor expressed on myeloid cells 1

other names :

triggering receptor expressed on myeloid cells 1 isoform 2; Triggering receptor expressed on myeloid cells 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1; CD_antigen: CD354

products gene name :

TREM1

other gene names :

TREM1; TREM1; CD354; TREM-1; TREM-1

uniprot entry name :

TREM1_HUMAN

reactivity :

Human

sequence length :

225

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TREM1. No significant cross-reactivity or interference between Human TREM1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 31.25pg/ml-2000pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.81 pg/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: The triggering receptor expressed on myeloid cells (TREM-1) is a recently identified activating receptor on neutrophil granulocytes (PMN), monocytes, and macrophage subsets. The expression of TREM-1 is upregulated by microbial products, that is, by toll-like receptor ligands such as lipoteichoic acid (LTA) of Gram-positive or lipopolysaccharide (LPS) of Gram-negative bacteria. Ligation of TREM-1 is synergistic with TLR agonists on the activation of receptor bearing cells for the release of inflammatory mediators like TNF-alpha and IL-8 and the initiation of neutrophil respiratory burst. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TREM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TREM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm } 2 nm. The concentration of TREM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

336088680

ncbi acc num :

NP_001229518.1

ncbi gb acc num :

NM_001242589.1

uniprot acc num :

Q9NP99

ncbi mol weight :

26,387 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway 106062!!DAP12 Interactions Pathway 685549!!Hemostasis Pathway 106028!!Immune System Pathway 106386!!Innate Immune System Pathway 106387

ncbi summary :

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

Function: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.2 PublicationsManual assertion based on experiment in:Ref.1

size :

96 Strip Wells

price :

670 USD

more info or order :

MyBioSource product webpage