Human Cholesterol 7alpha-Hydroxylase, CYP7A ELISA Kit | MyBioSource MBS701878 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cholesterol 7alpha-Hydroxylase, CYP7A ELISA Kit

catalog :

MBS701878

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701878

products type :

ELISA Kit

products full name :

Human Cholesterol 7alpha-Hydroxylase, CYP7A ELISA Kit

products short name :

cytochrome P450, family 7, subfamily A, polypeptide 1

products name syn :

Human Cholesterol 7alpha-Hydroxylase (CYP7A) ELISA Kit; CP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1; cytochrome P450; family 7; subfamily A; polypeptide 1

other names :

cholesterol 7-alpha-monooxygenase; Cholesterol 7-alpha-monooxygenase; cholesterol 7-alpha-monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1; cytochrome P450, family 7, subfamily A, polypeptide 1; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1

products gene name :

CYP7A1

other gene names :

CYP7A1; CYP7A1; CP7A; CYP7; CYPVII; CYP7

uniprot entry name :

CP7A1_HUMAN

reactivity :

Human

sequence length :

504

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CYP7A1. No significant cross-reactivity or interference between Human CYP7A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.7 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Cholesterol metabolism

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

166295200

ncbi acc num :

NP_000771.2

ncbi gb acc num :

NM_000780.3

uniprot acc num :

P22680

ncbi mol weight :

57,661 Da

ncbi pathways :

Bile Acid And Bile Salt Metabolism Pathway (106144); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (413432); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (468297); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Drug Induction Of Bile Acid Pathway (698755); Endogenous Sterols Pathway (105701); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

uniprot summary :

Function: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.1 PublicationManual assertion based on experiment in:Ref.8. Catalytic activity: Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.1 PublicationManual assertion based on experiment in:Ref.2. Cofactor: Heme group.1 PublicationManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage