ELISA/assay

Human S100 calcium binding protein P, S100P ELISA Kit

MBS701874

48-Strip-Wells

505 USD

ELISA Kit

Human S100 calcium binding protein P, S100P ELISA Kit

S100 calcium binding protein P

Human S100 calcium binding protein P (S100P) ELISA Kit; MIG9; S100 calcium-binding protein P; migration-inducing gene 9; S100 calcium binding protein P

protein S100-P; Protein S100-P; protein S100-P; protein S100-E; S100 calcium-binding protein P; migration-inducing gene 9 protein; S100 calcium binding protein P; Migration-inducing gene 9 protein; MIG9; Protein S100-E; S100 calcium-binding protein P

S100P

S100P; S100P; MIG9; S100E; MIG9

S100P_HUMAN

Human

95

This assay has high sensitivity and excellent specificity for detection of Human S100P. No significant cross-reactivity or interference between Human S100P and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100P has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100P present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100P is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100P bound in the initial step. The color development is stopped and the intensity of the color is measured.

5174663

NP_005971.1

NM_005980.2

P25815

10,400 Da

Gastric Cancer Network 1 Pathway (760635)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008]

Function: May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).3 PublicationsManual assertion based on experiment in:Ref.10

48-Strip-Wells

505 USD

96-Strip-Wells

715

5x96-Strip-Wells

2530

10x96-Strip-Wells

4730