catalog number :
MBS701854
products type :
ELISA Kit
products full name :
Rat Platelet Factor 4, PF-4 ELISA Kit
products short name :
[platelet factor 4]
products name syn :
[Rat Platelet Factor 4; PF-4 ELISA Kit; CXCL4; MGC138298; SCYB4; chemokine (C-X-C motif) ligand 4; platelet factor 4 (chemokine (C-X-C motif) ligand 4) ; platelet factor 4]
other names :
[Platelet factor 4; Platelet factor 4; C-X-C motif chemokine 4]
products gene name :
[PF4]
other gene names :
[PF4; CXCL4; SCYB4; PF-4]
uniprot entry name :
PLF4_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat PF4. No significant cross-reactivity or interference between Rat PF4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ug/ml-10 ug/ml. Sensitivity: 0.039 ug/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
3,318 Da
ncbi pathways :
CXCR3-mediated Signaling Events Pathway (138011); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)
uniprot summary :
Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells