catalog number :
MBS701847
products type :
ELISA Kit
products full name :
Mouse Vascular Endothelial cell Growth Factor C, VEGF-C ELISA Kit
products short name :
vascular endothelial growth factor C
products name syn :
Mouse Vascular Endothelial cell Growth Factor C; VEGF-C ELISA kit; Flt4-L; VRP; FLT4 ligand DHM; vascular endothelial growth factor-related protein; vascular endothelial growth factor C
other names :
vascular endothelial growth factor C; Vascular endothelial growth factor C; vascular endothelial growth factor C; VRP; flt4-L; flt4 ligand; vascular endothelial growth factor C isoform 129; vascular endothelial growth factor C isoform 184; vascular endothelial growth factor-related protein; vascular endothelial growth factor C; Flt4 ligand; Flt4-L; Vascular endothelial growth factor-related protein; VRP
products gene name :
VEGFC
other gene names :
Vegfc; Vegfc; VEGF-C; AW228853; VEGF-C; Flt4-L; VRP
uniprot entry name :
VEGFC_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse VEGF-C. No significant cross-reactivity or interference between mouse VEGF-C and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates and cell lysates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: 0.39 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-C bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033532.1
ncbi gb acc num :
NM_009506.2
ncbi mol weight :
46,471 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Heart Development Pathway (672437); Hemostasis Pathway (1001295); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); PI3K/Akt/mTOR Signaling Pathway (522982)
uniprot summary :
Function: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.2 PublicationsManual assertion based on experiment in:Ref.1
size4 :
10x96-Strip-Wells