Human 14-3-3 protein beta/alpha, YWHAB ELISA Kit | MyBioSource MBS701844 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 14-3-3 protein beta/alpha, YWHAB ELISA Kit

catalog :

MBS701844

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701844

products type :

ELISA Kit

products full name :

Human 14-3-3 protein beta/alpha, YWHAB ELISA Kit

products short name :

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide

products name syn :

Human 14-3-3 protein beta/alpha (YWHAB) ELISA kit; GW128; HS1; KCIP-1; 14-3-3 protein beta/alpha; brain protein 14-3-3; beta isoform; protein 1054; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta polypeptide

other names :

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; epididymis secretory protein Li 1; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

products gene name :

YWHAB

other gene names :

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1; KCIP-1

uniprot entry name :

1433B_HUMAN

reactivity :

Human

sequence length :

246

specificity :

This assay has high sensitivity and excellent specificity for detection of Human YWHAB. No significant cross-reactivity or interference between Human YWHAB and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 1.56 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAB bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507949

ncbi acc num :

NP_003395.1

ncbi gb acc num :

NM_003404.4

uniprot acc num :

P31946

ncbi mol weight :

28,082 Da

ncbi pathways :

ARMS-mediated Activation Pathway (106466); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Axon Guidance Pathway (105688); Butyrate Response Factor 1 (BRF1) Destabilizes MRNA Pathway (187213); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811)

ncbi summary :

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.2 PublicationsManual assertion based on experiment in:Ref.13

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage