catalog number :
MBS701841
products type :
ELISA Kit
products full name :
Mouse glial cell line-derived neurotrophic factor, GDNF ELISA Kit
products short name :
glial cell derived neurotrophic factor
products name syn :
Mouse glial cell line-derived neurotrophic factor; GDNF ELISA KIT; ATF1; ATF2; HFB1-GDNF; astrocyte-derived trophic factor; glial cell line derived neurotrophic factor; glial cell derived neurotrophic factor
other names :
glial cell line-derived neurotrophic factor isoform 1; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; mGDNF; astrocyte-derived trophic factor; glial cell line derived neurotrophic factor; Astrocyte-derived trophic factor; ATF
products gene name :
GDNF
other gene names :
Gdnf; Gdnf; AI385739; mGDNF; ATF
uniprot entry name :
GDNF_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse GDNF. No significant cross-reactivity or interference between mouse GDNF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.9 pg/ml -60 pg/ml. Sensitivity: The minimum detectable dose of mouse GDNF is typically less than 0.23 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034405.1
ncbi gb acc num :
NM_010275.3
ncbi mol weight :
23,662 Da
ncbi pathways :
Axon Guidance Pathway (970585); Developmental Biology Pathway (970813); NCAM Signaling For Neurite Out-growth Pathway (970782); NCAM1 Interactions Pathway (1000766); Spinal Cord Injury Pathway (739004)
uniprot summary :
Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
size4 :
10x96-Strip-Wells