Rat Acetylcholinesterase, AChE ELISA Kit | MyBioSource MBS701835 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Acetylcholinesterase, AChE ELISA Kit

catalog :

MBS701835

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701835

products type :

ELISA Kit

products full name :

Rat Acetylcholinesterase, AChE ELISA Kit

products short name :

acetylcholinesterase (Yt blood group)

products name syn :

Rat Acetylcholinesterase; AChE ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)

other names :

Acetylcholinesterase; Acetylcholinesterase

products gene name :

ACHE

other gene names :

ACHE; AChE

uniprot entry name :

ACES_RABIT

reactivity :

Rat

sequence length :

584

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat ACHE. No significant cross-reactivity or interference between Rat ACHE and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 7.8pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Neurotransmitter degradation

products description :

Introduction: In biochemistry, cholinesterase is an enzyme that catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Acetylcholinesterase(AChE), also known as RBC cholinesterase, erythrocyte cholinesterase, or (most formally) acetylcholine acetylhydrolase, found primarily in the blood and neural synapses. Acetylcholinesterase hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single AChE gene; and the structural diversity in the gene products arises from alternative mRNA splicing and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle, and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively-spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. Acetylcholinesterase is the target of nerve gases. The agents blocks the function of acetylcholinesterase and thus causes interminable muscle contraction throughout the body. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AChE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AChE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AChE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AChE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

2494388

ncbi acc num :

Q29499.1

uniprot acc num :

Q29499

ncbi mol weight :

64,630 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Monoamine Transport Pathway (198851); Neuronal System Pathway (106513); Neurotransmitter Clearance In The Synaptic Cleft Pathway (106526); Transmission Across Chemical Synapses Pathway (106516)

uniprot summary :

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Catalytic activity: Acetylcholine + H2O = choline + acetate.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage