catalog number :
MBS701833
products type :
ELISA Kit
products full name :
Human growth hormone releasing hormone, GHRH ELISA Kit
products short name :
growth hormone releasing hormone
products name syn :
Human growth hormone releasing hormone; GHRH ELISA Kit; GHRF; GRF; MGC119781; OTTHUMP00000030915; growth hormone releasing factor; sermorelin; somatocrinin; somatoliberin; growth hormone releasing hormone
other names :
somatoliberin isoform 2 preproprotein; Somatoliberin; somatoliberin; sermorelin; somatorelin; somatocrinin; growth hormone-releasing factor; growth hormone-releasing hormone; growth hormone releasing hormone; Growth hormone-releasing factor; GRF; Growth hormone-releasing hormone; GHRH; Somatocrinin; Somatorelin; INN: Sermorelin
products gene name :
GHRH
other gene names :
GHRH; GHRH; GRF; INN; GHRF; GHRF; GRF; GHRH
uniprot entry name :
SLIB_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human GHRH. No significant cross-reactivity or interference between Human GHRH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 20 pg/ml-400 pg/ml. Sensitivity: 8 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with biotin-BSA and Avidin. Standards and samples are pipetted into the wells with a biotin-conjugated antibody specific for GHRH. After washing, GHRH antibody conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRH in sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001171660.1
ncbi gb acc num :
NM_001184731.1
ncbi mol weight :
12,447 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
ncbi summary :
The protein encoded by this gene belongs to the glucagon family and is a preproprotein that is produced in the hypothalamus. The preproprotein is cleaved to form a 44 aa factor, also called somatocrinin, that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
size4 :
10x96-Strip-Wells