ELISA/assay

Human atrial natriuretic peptide, ANP ELISA Kit

MBS701832

48-Strip-Wells

510 USD

ELISA Kit

Human atrial natriuretic peptide, ANP ELISA Kit

natriuretic peptide precursor A

Human atrial natriuretic peptide (ANP) ELISA kit; ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptides A (NPPA) ; natriuretic peptide precursor A

natriuretic peptides A preproprotein; Natriuretic peptides A; natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A; natriuretic peptide A; CDD-ANF; PrepronatriodilatinCleaved into the following 2 chains:Cardiodilatin-related peptide; CDP; Atrial natriuretic factor; ANFAlternative name(s):Atrial natriuretic peptide; ANP

NPPA

NPPA; NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF; ANP; PND; CDP; ANF; ANP

ANF_HUMAN

Human

151

This assay has high sensitivity and excellent specificity for detection of Human NPPA. No significant cross-reactivity or interference between Human NPPA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, cell culture supernates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANP bound in the initial step. The color development is stopped and the intensity of the color is measured.

23510319

NP_006163.1

NM_006172.3

P01160

16,708 Da

Amyloids Pathway (366238); Disease Pathway (530764); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1 Signaling Pathway (695200); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (576276)

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.1 PublicationManual assertion based on experiment in:Ref.15

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800