ELISA/assay

Human Neurotensin, NT ELISA Kit

MBS701821

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Neurotensin, NT ELISA Kit

[neurotensin]

[Human Neurotensin; NT ELISA Kit; NMN-125; NN; NT; NT/N; NTS1; neuromedin N; neurotensin/neuromedin N; neurotensin]

[neurotensin/neuromedin N preproprotein; Neurotensin/neuromedin N; neurotensin/neuromedin N; pro-neurotensin/neuromedin; neurotensin; NmN-125]

[NTS]

[NTS; NTS; NN; NT; NT/N; NTS1; NMN-125; NN; NmN; NT]

NEUT_HUMAN

Human

170

This assay has high sensitivity and excellent specificity for detection of human NT. No significant cross-reactivity or interference between human NT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, tissue homogenates and cell lysates. Detection Range: 15.6 pg/ml -1000 pg/mL . Sensitivity: <3.9 pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

5453816

NP_006174.1

NM_006183.4

P30990

19,795 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a common precursor for two peptides, neuromedin N and neurotensin. Neurotensin is a secreted tridecapeptide, which is widely distributed throughout the central nervous system, and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events, in the maintenance of gut structure and function, and in the regulation of fat metabolism. Tissue-specific processing may lead to the formation in some tissues of larger forms of neuromedin N and neurotensin. The large forms may represent more stable peptides that are also biologically active. [provided by RefSeq, Jul 2008]

Function: Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145