Human permeability glycoprotein, P-gp/ABCB1 ELISA Kit | MyBioSource MBS701812 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human permeability glycoprotein, P-gp/ABCB1 ELISA Kit

catalog :

MBS701812

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701812

products type :

ELISA Kit

products full name :

Human permeability glycoprotein, P-gp/ABCB1 ELISA Kit

products short name :

ATP-binding cassette, sub-family B (MDR/TAP) , member 1

products name syn :

Human permeability glycoprotein; P-gp/ABCB1 ELISA Kit; ABC20; CD243; CLCS; GP170; MDR1; MGC163296; P-GP; PGY1; ATP-binding cassette; subfamily B; member 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; multidrug resistance protein 1; ATP-binding cassette; sub-family B (MDR/TAP); member 1

other names :

multidrug resistance protein 1; Multidrug resistance protein 1; multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD_antigen: CD243

products gene name :

ABCB1

other gene names :

ABCB1; ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; MDR1; PGY1

uniprot entry name :

MDR1_HUMAN

reactivity :

Human

sequence length :

1280

specificity :

This assay has high sensitivity and excellent specificity for detection of human ABCB1. No significant cross-reactivity or interference between human ABCB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, ascitic fluid, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human ABCB1 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABCB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCB1 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for ABCB1 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

42741659

ncbi acc num :

NP_000918.2

ncbi gb acc num :

NM_000927.4

uniprot acc num :

P08183

ncbi mol weight :

141,479 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); Abacavir Transmembrane Transport Pathway (645350); Abacavir Transport And Metabolism Pathway (645349); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Codeine And Morphine Metabolism Pathway (198831); Drug Induction Of Bile Acid Pathway (698755); HIF-1-alpha Transcription Factor Network Pathway (138045)

ncbi summary :

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Catalytic activity: ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage