catalog number :
MBS701806
products type :
ELISA Kit
products full name :
Mouse Tubulin Beta-3 Chain, TUBB3 ELISA Kit
products short name :
tubulin, beta 3
products name syn :
Mouse Tubulin Beta-3 Chain (TUBB3) ELISA Kit; MC1R; TUBB4; beta-4; tubulin; beta; 4; tubulin; beta 3
other names :
tubulin beta-3 chain; Tubulin beta-3 chain; tubulin beta-3 chain; betaIII-tubulin; tubulin, beta 3 class III
products gene name :
TUBB3
other gene names :
Tubb3; Tubb3; M(beta)3; M(beta)6; 3200002H15Rik
uniprot entry name :
TBB3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TUBB3. No significant cross-reactivity or interference between mouse TUBB3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates, cell lysates. Assay Type: Sandwich. Detection Range: 0.625ng/ml-40 ng/ml. Sensitivity: Typically less than 0.156 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TUBB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TUBB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_075768.1
ncbi gb acc num :
NM_023279.2
ncbi mol weight :
50,419 Da
ncbi pathways :
Chaperonin-mediated Protein Folding Pathway (1000366); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (1000367); Diurnally Regulated Genes With Circadian Orthologs Pathway (198406); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (1000369); Gap Junction Pathway (83269); Gap Junction Pathway (483); Metabolism Of Proteins Pathway (970583); Phagosome Pathway (153914); Phagosome Pathway (153859); Post-chaperonin Tubulin Folding Pathway (1000372)
uniprot summary :
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.1 PublicationManual assertion based on experiment in:Ref.7
size4 :
10x96-Strip-Wells