catalog number :
MBS701792
products type :
ELISA Kit
products full name :
Mouse Macrophage Inflammatory Protein 1alpha, MIP-1alpha ELISA Kit
products short name :
chemokine (C-C motif) ligand 3
products name syn :
Mouse Macrophage Inflammatory Protein 1alpha; MIP-1alpha ELISA kit; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; LD78 alpha beta; small inducible cytokine A3 (homologous to mouse Mip-1a) ; chemokine (C-C motif) ligand 3
other names :
C-C motif chemokine 3; C-C motif chemokine 3; C-C motif chemokine 3; TY-5; L2G25B; MIP1 (a); SIS-alpha; MIP-1 alpha; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; heparin-binding chemotaxis protein; macrophage inflammatory protein-1alpha; macrophage inflammatory protein 1-alpha; chemokine (C-C motif) ligand 3; Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5
products gene name :
CCL3
other gene names :
Ccl3; Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha; Mip1a; Scya3; MIP-1-alpha
uniprot entry name :
CCL3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CCL3. No significant cross-reactivity or interference between Mouse CCL3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 23.5 pg/ml-1500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 5.9 pg/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis
products description :
For Sample For the quantitative determination of mouse macrophage inflammatory protein 1? (MIP-1?) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-1? bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035467.1
ncbi gb acc num :
NM_011337.2
ncbi mol weight :
10,345 Da
ncbi pathways :
Chagas Disease (American Trypanosomiasis) Pathway 147810!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Chemokine Receptors Bind Chemokines Pathway 1000944!!Chemokine Signaling Pathway 99271!!Chemokine Signaling Pathway 672435!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!GPCR Ligand Binding Pathway 1000941
uniprot summary :
Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.